Hydractinia Genome Portal
|Dataset Name||Description||Origin of Material||Link to Raw Reads||Link to Assembly||Publication(s)|
|Polyp-specific||Dataset includes four replicated libraries of feeding (nonreproductive) polyps (gastrozooids), two replicated libraries of female reproductive polyps (gonozooids), two replicated libraries of male reproductive polyps (gonozooids), and four replicated libraries of defensive (nonreproductive) polyps (dactylozooids).||Hydractinia symbiolongicarpus colonies collected in Woods Hole MA and purchased from Marine Biological Laboratories||SRX474462||GAWH00000000||Sanders, et al. (2014) BMC Genomics
Sanders, et al. (2015) Genome Biology and Evolution
|Polyp-specific||This project seeks to identify differentially expressed genes between different polyp and sex types in the colonial hydrozoan Hydractinia symbiolongicarpus. Data included in this deposition include multiple replicate RNAseq datasets from male gonozoids, male gastrozoids, female gonozoids and female gastrozoids. Data were obtained using the HiSeq2000 platform||Hydractinia symbiolongicarpus colonies collected from Barnstable Harbor, MA, USA in July 2010||PRJNA244078||Data Files on Dryad||Plachetzki et al. (2014) Integrative & Comparative Biology|
Validated Procedures Key
E = Elisa
FC = Flow Cytometry
IF = Immunofluorescence
IP = Immunoprecipitation
WB = Western Blot
O = Other
Please visit the Get Started page to view the protocols regarding Hydractinia husbandry.
- Cesium Chloride
- Whole tissue dissociation
- Embryo dissociation
- DNA/RNA extraction
As 3D printing has become more affordable, it has proven useful for customizing tools. We have included links to some commonly printed objects used in Hydractinia labs.
- Hydractinia breeder box
- Hydractinia viewing dish with integrated slide holder
- Hydractinia rack
- Dissection scope phone mount
To design tools, there are many resources available. For beginners, one free tool for designing is Tinkercad by Autodesk. Sign up for an account and complete the relevant lessons for using Tinkercad, then start desinging your own objects to fit your project. Files can be downloaded in various formats, though most commonly .stl or .obj files are used.
To prepare objects for printing, the object (.stl or .obj) must be separated or “sliced” into the layers that the printer will use to print the object. One free software that can be utilized is Ultimaker Cura. This will produce a .gcode file that 3D printers use to print the object.
For 3D printers, there are many options. For beginners, an affordable model that the Nicotra Lab has used to successfully print objects is the Creatlity Ender3. A second and more expensive model that has been used to successfully print objects is the Prusa i3 MK3. Filament can be purchased from various vendors. The Nicotra Lab has purchased filament from HatchBox.
To find additional reserach-related objects or to share your objects with the Hydractinia community, there is a group on Thingiverse called Hydractinia. If you have any objects that you think should be added to the quick links above, contact Matt.
Other Protocols and Resources
- Coming soon, some protocols will be hosted on Protocols.io.
- About Hydractinia (anatomy atlas, development timetable, life cycle)
Stay tuned for future resources such as cryopreserved samples, newletters, job postings, forums, and more! Let us know if you have suggestions.
Frequently Asked Questions
Coming soon, frequently asked questions.